Foregene RT EasyTM II Master Premix for first-strand cDNA synthesis for Real Time PCR

Description:

5×RO EasyTM Mix contains Foregene Reverse Transcriptase specially developed by Foregene, which is a new reverse transcriptase with stronger RNA affinity. The optimized system enables a faster reverse transcription rate, and first-strand cDNA synthesis can be completed within 15 minutes at 42°C.
RT EasyTM II (with gDNase) is a reverse transcription system specially developed for real-time PCR to quickly remove genomic DNA contamination. 5×gDNase Mix can quickly remove the remaining genome in the RNA at 42°C for 2 minutes, effectively avoiding the interference of the genome on the qPCR results.
The reverse transcription system of the kit can also maintain good activity at 50°C. Therefore, it is more beneficial for complex RNA templates, and RNA templates with high GC content and complex secondary structure can be easily transcribed.

Features and Benefits:
- Ability to efficiently remove gDNA, which can remove gDNA from the template within 2 minutes.
-Efficient reverse transcription system, it only takes 15 minutes to complete the synthesis of first-strand cDNA.
- Complex templates: templates with high GC content and complex secondary structures can also be reversed efficiently.
- High-sensitivity reverse transcription system, pg-level templates can also obtain high-quality cDNA.
- The reverse transcription system has high thermal stability, the optimum reaction temperature is 42°C, and it still has good reverse transcription performance at 50°C.

Kit applications:

Used directly in Real Time PCR to quantitatively analyze gene expression.

It can quickly and accurately analyze trace RNA such as RNA virus.

Reverse transcription of RNA templates with high GC content or complex secondary structure.

Specifications:

25×20µl rxns,100×20µl rxns

Kit components:

Work Flow:

Storage and Shelf life:

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.